Sustainable Construction: Optimization of Microbial Soil Stabilization for Enhanced Construction Materials

Authors

DOI:

https://doi.org/10.5281/zenodo.14874393

Keywords:

Artificial Rock Conglomerate, Microbially Induced Calcium Precipitation, Sand Stabilization, Urease Activity

Abstract

Microbially Induced Calcium Precipitation (MICP) has emerged as an effective and sustainable method for soil stabilization, utilizing microbial processes to enhance soil strength through calcium carbonate formation. This research explores the transformation of Mongu sand into a strong construction material using MICP, with a focus on optimizing urease activity, bacterial concentration, and cementation media. Given the scarcity of rock conglomerates in the region but the abundance of sand, this study provides innovative solutions to address this issue by improving and even transforming materials into more suitable forms for construction. MICP presents significant potential for infrastructure development in areas with limited access to conventional materials, with future efforts aimed at scaling the process for larger and more complex projects. The findings provide insights into the optimal parameters for material transformation, revealing that maintaining urease activity between 10-15 µmol urea hydrolyzed per minute per mg of protein and bacterial concentrations of 1.5 x 10⁸ CFU/mL results in a substantial improvement in compressive strength and durability—up to 475% higher than untreated sand. Additionally, the study underscores the effectiveness of Sporosarcina pasteurii in promoting calcium carbonate precipitation, further emphasizing MICP’s potential for soil stabilization and construction in resource-constrained regions.

Downloads

Download data is not yet available.

References

Michelo, S., Kunda, S., & Musonda, D. (2018). Geotechnical assessment of sands from Western Zambia for construction applications. International Journal of Geotechnical Engineering, 10(4), 299-310.

S. Tunay. (2020). The effect of bacterial concentration on calcium carbonate precipitation in soils. Journal of Biological Engineering, 14(1).

Whiffin, V. S., van Paassen, L. A., & Harkes, M. P. (2007). Microbial carbonate precipitation as a soil improvement technique. Geomicrobiology Journal, 24(5), 417-423.

Stocks-Fischer, S., Galinat, J. K., & Bang, S. S. (1999). Microbiological precipitation of CaCO₃. Soil Biology and Biochemistry, 31(11), 1563-1571.

DeJong, J. T., Mortensen, B. M., Martinez, B. C., & Nelson, D. C. (2010). Bio-mediated soil improvement. Ecological Engineering, 36(2), 197-210.

Akiyama, M., & Kawasaki, S. (2012). Microbially mediated sand solidification using calcium carbonate. Proceedings of the ICE - Ground Improvement, 165(4), 229-238.

Dhami, N. K., Reddy, M. S., & Mukherjee, A. (2013). Biomineralization of calcium carbonates and their engineered applications: A review. Frontiers in Microbiology, 4, 314.

DeJong, J. T., Soga, K., Banwart, S. A., Whalley, W. R., Ginn, T. R., Nelson, D. C., & Aydilek, A. H. (2011). Soil engineering in vivo: Harnessing natural biogeochemical systems for sustainable, multi-functional engineering solutions. Journal of the Royal Society Interface, 8(54), 1-15.

Ndilila, W., Chileshe, M.N., & Mulenga, C.K. (2017). Geotechnical properties of sands from the Western Province of Zambia. Journal of African Earth Sciences, 135, 112-121.

Qian, C., & Cheng, L. (2017). Engineering properties of MICP-treated sand under different environmental conditions. Construction and Building Materials, 142, 316-324.

Achal, V., Mukherjee, A., Basu, P. C., & Reddy, M. S. (2009). Strain improvement of Sporosarcina pasteurii for enhanced urease and calcite production. Journal of Industrial Microbiology & Biotechnology, 36(7), 981-988.

Achal, V., Mukherjee, A., & Reddy, M. S. (2013). Biogenic treatment improves the durability and remediates the cracks of concrete structures. Construction and Building Materials, 48, 1-5.

Cheng, L., & Cord-Ruwisch, R. (2013). Selective enrichment and production of highly urease active bacteria by non-sterile chemostat culture. Journal of Applied Microbiology, 114(3), 713-725.

Whiffin, V. S. (2004). Microbial CaCO₃ precipitation for the production of biocement. Murdoch University.

Cheng, L., & Cord-Ruwisch, R. (2012). In situ soil cementation with ureolytic bacteria to improve soil mechanical properties. Journal of Geotechnical and Geoenvironmental Engineering, 138(3), 333-340.

Chou, C. W., Seagren, E. A., Aydilek, A. H., & Lai, M. (2011). Biocalcification of sand through ureolysis. Journal of Geotechnical and Geoenvironmental Engineering, 137(12), 1179-1189.

van Paassen, L. A., Harkes, M. P., van Zwieten, G. A., van der Zon, W. H., van der Star, W. R., & van Loosdrecht, M. C. (2010). Scale up of BioGrout: A biological ground reinforcement method. Journal of Environmental Geotechnics, 1(2), 123-138.

Gomez, M.G., Anderson, C.M., Graddy, C.M.R., DeJong, J.T., Nelson, D.C., & Ginn, T.R. (2017). Large scale biocementation field trials in sands: lessons learned. Journal of Geotechnical and Geoenvironmental Engineering, 145(5).

Carmona, J. S., Oliveira, P. J. V., & Monteiro, G. A. (2016). Enhancement of sand properties using biocementation via microbially induced calcite precipitation. Soils and Rocks, 39(3), 321-330.

Cheng, L., Shahin, M.A., & Cord-Ruwisch, R. (2014). Cementation of sand soil by microbially induced calcite precipitation at various degrees of saturation. Canadian Geotechnical Journal, 51(8), 917-924.

Fujita, Y., Taylor, J. L., Gresham, T. L. T., Delwiche, M. E., Colwell, F. S., & McLing, T. L. (2008). Stimulation of microbial urea hydrolysis in groundwater to enhance calcite precipitation. Environmental Science and Technology, 42(8), 3025-3032.

Qian, C., Wang, R., Cheng, L., & Wang, J. (2011). Application of microbial carbonate precipitation in self-healing of cement-based materials. Journal of Materials in Civil Engineering, 23(7), 992-997.

Cheng, L., & Cord-Ruwisch, R. (2013). Upscaling effects of soil improvement by microbially induced calcite precipitation by surface percolation. Geomicrobiology Journal, 30(5), 461-469.

Khan, A., & Khandaker, M.U. (2020). Effects of environmental factors on the calcite precipitation induced by ureolytic bacteria. Environmetal Technology and Innivation, 17.

Cheng, L., & Cord-Ruwisch, R. (2012). In situ soil cementation with ureolytic bacteria by surface percolation. Ecological Engineering, 42, 64-72.

Katotobwe, C., Chabota K., & Agabu, K.L. (2024). Enhanced local contractor participation: case of Zambia water supply and sanitation sub-sector. International Journal of Engieering and Management Research, 14(1), 246-260d.

Published

2025-02-09
CITATION
DOI: 10.5281/zenodo.14874393
Published: 2025-02-09

How to Cite

Shikabonga, J., Lungu, K. A., Mwaba, D., Mubita, C., Mulenga, M. N., & Mwanaumo, E. (2025). Sustainable Construction: Optimization of Microbial Soil Stabilization for Enhanced Construction Materials. International Journal of Engineering and Management Research, 15(1), 36–52. https://doi.org/10.5281/zenodo.14874393

Similar Articles

<< < 7 8 9 10 11 12 13 > >> 

You may also start an advanced similarity search for this article.